Sodium bicarbonate loading limits tubular cast formation independent of glomerular injury and proteinuria in Dahl salt-sensitive rats

• DOI: 10.1042/cs20171630
• Publication Date: 2018-04-12T17:30:32Z
• Authors: Sarah C. Ray; Bansari Patel; Debra L. Irsik; Jingping Sun; Hiram Ocasio; Gene R. Crislip; Chunhua H. Jin; JianKang Chen; Babak Baban; Aaron J. Polichno...; Paul M. O’Connor
• Keywords: Glomerulosclerosis; Bicarbonate; Renal Injury

Sodium bicarbonate (NaHCO3) slows the decline in kidney function patients with chronic disease (CKD), yet mechanisms mediating this effect remain unclear. The Dahl salt-sensitive (SS) rat develops hypertension and progressive renal injury when fed a high salt diet; however, of alkali loading on has never been investigated model. We hypothesized that NaHCO3 protects from development rats via luminal alkalization which limits formation tubular casts, are prominent pathological feature To examine hypothesis, we determined blood pressure responses SS drinking vehicle (0.1 M NaCl) or M) solutions as well lacking voltage-gated proton channel (Hv1). found oral reduced NH4+ production, cast formation, interstitial fibrosis diet for 2 weeks. This was independent changes pressure, glomerular injury, proteinuria did not associate inflammatory status. null mutation Hv1 also limited injury. As is localized to membrane TAL, our data suggest fluid within segment Reduced secondary TAL segments may mediate some protective effects observed CKD patients.