The pseudogene PTENP1 regulates smooth muscle cells as a competing endogenous RNA

• DOI: 10.1042/cs20190156
• Publication Date: 2019-06-24T20:54:24Z
• Authors: Yanxian Lai; Jianyong Li; Lintao Zhong; Xiang He; Xiaoyun Si; Yili Sun; Yanmei Chen; Jiayuan Zhong; Yinlan Hu; Bing Li; Wangjun Liao; Cheng Liu; Yulin Liao; Jiancheng Xiu; Jianping Bin
• Keywords: Tensin; Competing Endogenous RNA

The long non-coding RNA (lncRNA) PTENP1 is a pseudogene of phosphatase and tensin homologue deleted on chromosome ten (PTEN), has been implicated in smooth muscle cell (SMC) proliferation apoptosis. the PTEN. However, it unclear whether how functions apoptosis human aortic SMCs (HASMCs). Here, we hypothesised that inhibits HASMC enhances by promoting PTEN expression. PCR analysis Western blot assays respectively showed both were up-regulated dissection (AD) samples. overexpression significantly increased protein expression PTEN, promoted inhibited HASMCs. silencing exhibited opposite effects mitigated H2O2-induced In an angiotensin II (Ang II)-induced mouse aneurysm (AA) model, potentiated SMC apoptosis, exacerbated formation. Mechanistically, pull-down assay series luciferase reporter using miR-21 mimics or inhibitors identified as molecular sponge for to endogenously compete binding between transcript, releasing This finding was further supported vitro immunofluorescent evidence showing decreased upon mimic administration under baseline overexpression. Ex vivo rescue induced Finally, substantially reduced Akt phosphorylation cyclin D1 E levels with Our study mediator homeostasis suggests potential target AD AA intervention.