Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes

• DOI: 10.1042/cs20201145
• Publication Date: 2021-01-26T00:06:39Z
• Authors: Rui Zhou; Changpei Xiang; Guangzhao Cao; He Xu; Yi Zhang; Hongjun Yang; Jingjing Zhang
• Keywords: HaCaT; MMP9

The high disability, mortality and morbidity of diabetic ulcers make it urgent to explore effective strategies for wound repair. TrxR1 plays a vital role in regulating redox homeostasis various pathologies. In the present study, effect berberine (BBR) on wounds was investigated streptozotocin (STZ)-induced rats glucose (HG)-induced cell model, mechanism BBR elucidated. treatment remarkably accelerated healing enhanced extracellular matrix (ECM) synthesis significantly inhibited HG-induced HaCaT damage. Further analysis indicated that activated TrxR1, suppressed its downstream JNK signaling, thereby inhibiting oxidative stress apoptosis, promoted proliferation, down-regulated metalloproteinase (MMP) 9 (MMP9) up-regulated transforming growth factor-β1 (TGF-β1) tissue inhibitors MMP 1 (TIMP1), resulting healing. Importantly, enhancement repair further abolished by inhibitor. Moreover, induced combination STZ injection high-fat diet, increased closure rate expression, this reversed These data topical activating TrxR1. Targeting may be novel, strategy restoring promoting