Expression of IL‐8 by cells of the odontoblast layer in vitro

• DOI: 10.1046/j.0909-8836.1999.eos107209.x
• Publication Date: 2003-11-21T10:00:38Z
• Authors: Linda G. Levin; Andrew Rudd; Athanasia Bletsa; Howard Reisner
• Keywords: Chapel

Due to their peripheral location in the dental pulp and cellular extension into dentin, odontoblasts are first pulpal cells encounter pathogens. The association of with immunoglobulins dendritic during microbial invasion dentin implies that these may possess a role innate adaptive immune responses, however this has not been examined. A pivotal step response is detection foreign antigen recruitment effector area. IL-8 potent chemotactic cytokine plays an important inflammatory response. purpose study was determine if capable expressing pro-inflammatory chemokine IL-8. Human from intact, noncarious third molars were maintained culture exposed Escherichia coli lipopolysaccharide (LPS) (serotype 055:B5) on day 4 for 8-10 h humidified 5% CO2 incubator. Control experimental samples assayed by reverse transcription-polymerase chain reaction (RT-PCR) Western blot production mRNA protein. Analysis PCR products revealed odontoblast layer model constitutively expressed low levels IL-8, which increased E. LPS exposure. blotting confirmed translated These results imply producing mediators, thereby actively participating neutrophils bacterial by-products.