Expression of HER‐2/neu gene and protein in salivary duct carcinomas of parotid gland as revealed by fluorescence in‐situ hybridization and immunohistochemistry

• DOI: 10.1046/j.1365-2559.2003.01600.x
• Publication Date: 2004-12-25T02:00:06Z
• Authors: A Skálová; I Stárek; T Vanecek; V Kucerová; L Plank; P Szépe; S Di Palma; I Leivo
• Keywords: Salivary duct carcinoma; Salivary gland cancer; HER2/neu

Salivary duct carcinoma is a highly malignant salivary gland tumour with aggressive clinical behaviour, characterized by histological resemblance to invasive ductal of the breast. Amplification HER-2/neu oncogene and over-expression its gene product have both prognostic therapeutic implications in breast cancer. Recent report on carcinomas for using immunohistochemistry (IHC) has shown most cases. However, correlation between IHC molecular genetic analysis not yet been performed.We now evaluated 11 cases status fluorescent in-situ hybridization (FISH). To our knowledge, this first carcinoma.In immunohistochemistry, protein was identified as distinct membrane staining cells all cases, while only four revealed an amplification means FISH analysis. Both amplified non-amplified strong immunohistochemical were associated poor outcome patients. Apparently, could also be controlled mechanisms other than amplification. In group tumours carcinoma, detected three ex pleomorphic adenoma. Thus, useful marker transformation adenomas.