Relation between distribution of viral RNA and development of histopathological changes in encephalomyocarditis virus‐induced orchitis in mice

• DOI: 10.1046/j.1365-2613.1996.959097.x
• Publication Date: 2003-11-02T15:19:46Z
• Authors: AITO UENO; MAKIO TAKEDA; KENSUKE HIRASAWA; SHIN’ICH ITAGAKI; KUNIO DOI
• Keywords: Orchitis

The relation between the distribution of viral RNA and development histopathological changes was investigated in early stage encephalomyocarditis (EMC) virus-induced orchitis mice. Signals were first detected by situ hybridization a few Sertoli cells almost intact germinal epithelia at 2 days post-inoculation (d.p.i.). number bearing signals increased 3 d.p.i. when mild degenerative exceptionally found epithelia. came to be not only but also small spermatogonia 4 moderate developed epithelia, resulting desquamation degenerated cells. At same time, virus-like particles observed electron-microscopy desquamated after 5 d.p.i., luminal obstruction with cellular debris inflammatory generally found. These results suggest that EMC virus carried seminiferous tubules via blood attacks then damages spermatogonia.