Transcript profiles and deduced changes of metabolic pathways in maternal and filial tissues of developing barley grains

• DOI: 10.1046/j.1365-313x.2003.01981.x
• Publication Date: 2004-12-29T19:37:12Z
• Authors: Nese Sreenivasulu; Lothar Altschmied; Volodymyr Radchuk; Sabine Gubatz; Ulrich Wobus; Winfriede Weschke
• Keywords: Caryopsis

Different aspects of barley grain development have been studied in detail, but a more global analysis gene expression patterns is still missing. We employed macro arrays, containing 1184 unique sequences from 1421 cDNA fragments, to study profiles maternal and filial tissues developing caryopses fertilization early storage phase. Principle component (PCA) defined distinct networks the pre-storage (0, 2, 4 days after flowering (DAF)) phase (10 12 DAF). During an intermediate (6 8 DAF), PCA visualizes dramatic re-programming transcriptional machinery. In tissues, large set protein-mobilizing enzyme mRNAs, together with upregulated lipid-mobilizing downregulated reactive oxygen species (ROS)-scavenging genes, suggests mobilization stored compounds programmed cell death (PCD). tissue fraction, genes highly expressed during involved growth processes, including wall biosynthesis. The data suggest that necessary UDP-glucose provided both by sucrose synthase (isoform 3) invertase-driven pathway. Further, major developmental changes pathways producing energy are predicted. A bell-shaped profile peak characteristic for associated photosynthesis ATP production. photosynthesis-determined increase concentration could be prerequisite initiation filling, dominated starch protein synthesis. Storage product accumulation accompanied high activity glycolysis fermentation, as well citric acid cycle.