Immunomodulating Effect of Blood Transfusion: Is Storage Time Important?

• DOI: 10.1046/j.1423-0410.1998.7430176.x
• Publication Date: 2004-12-29T22:39:34Z
• Authors: Tommie Mynster; Esben Dybkjœr; Gitte Kronborg; Hans Jørgen Nielsen
• Keywords: Buffy coat; Blood bank; Blood cell

Abstract Objectives: TNF‐α and IL‐2 are important cytokines in macrophage T‐lymphocyte activity against infection dissemination of malignant cells. We studied the influence supernatants from stored whole blood buffy‐coat‐depleted SAGM (saline, adenine, glucose mannitol) stimulating release an ex vivo assay. Methods: Supernatants 10 units were collected after 1, 21 35 days standard bank storage. Heparinized 20 healthy volunteers (as ‘recipients’), corresponding ABO Rh type to blood, used a culture system with LPS PHA as stimulators release. The effect added supernatants, either or on cytokine was evaluated compared saline control. concentration analyzed by ELISA for 24 h 72 h, respectively. Results: both showed significant decrease LPS‐ PHA‐stimulated that dependent storage time. not detected response stimulation. significantly reduced related time blood. Conclusions: Recipient induced transfusion seems be This may have implications transfusion‐induced immune modulation.