Chemical structures and immunolocalization of glycosphingolipids isolated from Diphyllobothrium hottai adult worms and plerocercoids

• DOI: 10.1046/j.1432-1033.2002.03041.x
• Publication Date: 2003-03-11T18:32:52Z
• Authors: Hideyuki Iriko; Kazuo Nakamura; Hisako Kojima; Naoko Iida‐Tanaka; Takeshi Kasama; Yasushi Kawakami; Ineo Ishizuka; Akihiko Uchida; Yoshihiko Murata; Yoichi Tamai
• Keywords: Glycosphingolipid; Immunostaining; Silicic acid; Sphingolipid

Glycosphingolipids (GSLs) were purified from adults and plerocercoids of the tapeworm Diphyllobothrium hottai, their chemical structures determined. Total lipid fractions prepared chloroform/methanol extracts whole tissues fractionated successively on ion-exchange chromatography, silicic acid column preparative TLC. The GSLs characterized by methylation analysis, TLC-immunostaining, liquid secondary ion MS, MALDI-TOF 1H-NMR. Ten isolated adult worms four plerocercoids, comprising mono-, di-, tri-, tetra-, pentasaccharides. GSL Gal beta 1-4(Fuc alpha 1-3)Glc 1-3Gal 1-Cer was found in but not whereas Ga lbeta 1-4 (Fuc 1-3(Gal 1-6)Gal both plerocercoids. We previously a similar series cestode Spirometra erinaceieuropaei, termed them 'spirometosides'[Kawakami, Y. et al. (1996) Eur J. Biochem. 239, 905-911]. core structure spirometosides, 1-4Glc 1-3 1-Cer, may have taxonomic significance, being characteristic pseudophyllidean tapeworms. In present study, compositions significantly different between growth-dependent changes composition documented. novel dihexosylceramide, Glc which is possible precursor for spirometosides. Immunohistochemical examination showed that spirometoside are highly enriched inner surface bothria, major point contact worm host's intestine. Our findings indicate spirometosides involved host-parasite interaction.