Structure of an acidic exopolysaccharide produced by the diazotrophic endophytic bacterium Burkholderia brasiliensis

• DOI: 10.1046/j.1432-1327.2001.02196.x
• Publication Date: 2003-03-11T18:09:49Z
• Authors: Katherine A. Mattos; Christopher Jones; Norton Heise; José O. Previato; Lúcia Mendonça‐Pr...
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Magnetic Resonance Spectroscopy; Carbohydrate Sequence; Molecular Structure; Burkholderia; Molecular Sequence Data; Polysaccharides, Bacterial; Methylation; Gas Chromatography-Mass Spectrometry

The structure of an exopolysaccharide (EPS) produced by Burkholderia brasiliensis , a diazotrophic endophytic organism originally isolated from rice roots, has been determined. bacterium was grown in synthetic medium, containing mannitol and glutamate, which favours the expression two anionic EPSs, were separated anion‐exchange chromatography. repeat unit EPS A, eluted at higher ionic strength, determined combination methylation analysis, partial hydrolysis, chemical degradations, NMR spectroscopic studies, shown to be linear O ‐acetylated pentasaccharide: →4)‐α‐ d ‐Glc p ‐(1→2)‐α‐ l ‐Rha ‐(1→4)‐α‐ A‐(1→3)‐β‐ [2OAc]‐(1→4)‐β‐ ‐(1→