Levels of IL‐6 and soluble IL‐6 receptor are increased in HIV patients with a history of immune restoration disease after HAART

• DOI: 10.1046/j.1464-2662.2001.00096.x
• Publication Date: 2003-03-12T00:19:56Z
• Authors: SF Stone; P Price; NM Keane; RJ Murray; MA French
• Keywords: Adult; CD4-Positive T-Lymphocytes; Male; Interleukin-6; HIV; Enzyme-Linked Immunosorbent Assay; HIV Infections; Middle Aged; Flow Cytometry; Receptors, Interleukin-6; Statistics, Nonparametric; 3. Good health; Interferon-gamma; 03 medical and health sciences; 0302 clinical medicine; Antiretroviral Therapy, Highly Active; Humans; RNA, Viral; Female

Objectives We have previously described immune restoration diseases (IRD) associated with asymptomatic opportunistic infections presenting in immunodeficient HIV patients responding to highly active antiretroviral therapy (HAART). Here we address the question of whether a history IRD exhibit persistent activation, shown by elevated levels interleukin‐(IL)‐6 and soluble IL‐6 receptor (sIL‐6R). Methods Peripheral blood mononuclear cells (PBMCs) plasma were collected from nadir CD4 T cell counts < 80/µL who had achieved reconstitution after HAART ( n =14) or without =15) experiencing IRD, severely (SID) 80 cells/µL =8) seronegative controls =15). PBMC production IL‐6, sIL‐6R interferon (IFN)‐γ (PBMC only) measured enzyme linked immunosorbent assay (ELISA). Intracellular flow cytometry was used determine predominant cellular source controls. Results Unstimulated produced significantly higher amounts than non‐IRD The concentration also supernatants mitogen‐stimulated compared patients. IFN‐γ did not differ between patients, SID Monocytes both Conclusions Patients sIL‐6R.