Activation of Protein Kinase C by Trimethyltin: Relevance to Neurotoxicity

• DOI: 10.1046/j.1471-4159.1995.65052338.x
• Publication Date: 2010-07-15T23:54:40Z
• Authors: Goran Pavlaković; Michael D. Kane; Charles L. Eyer; Anumantha Kanthasamy; Gary E. Isom
• Keywords: Chelerythrine; Neurotoxicity; Phorbol

Abstract: The differentiated PC12 cell neuronal model was used to determine the effect of trimethyltin (TMT) on protein kinase C (PKC). Cells treated with 5–20 µ M TMT showed a partial and sustained PKC translocation within 30 min persisted over 24‐h period. treatment accompanied by low level down‐regulation 24 h, which small compared that produced phorbol esters. Confocal imaging cells translocates plasma membrane is blocked inhibitor chelerythrine (1 ). Phorbol myristate‐induced or inhibition provided protection against TMT‐induced cytotoxicity. It concluded activation contribute cytotoxicity in cells.