Functional Glucokinase Isoforms Are Expressed in Rat Brain

• DOI: 10.1046/j.1471-4159.2000.0741848.x
• Publication Date: 2003-03-12T06:45:16Z
• Authors: Isabel Roncero; Elvira Alvarez; Patricia Vázquez; Enrique Blázquez
• Keywords: Glucokinase

Abstract: Recently, the description of glucokinase mRNA in certain neuroendocrine cells has opened new ways to characterize this enzyme rat brain. In study, we found and a similar RNA splicing pattern gene product hypothalamus pancreatic islets; that codes for B1 isoform was most abundant, with minor amounts those coding B2, P1, P2, P1/B2, P2/B2 isoforms. Glucokinase expression brain gave rise protein 52 kDa high apparent K m glucose no inhibition by 6‐phosphate, contribution total phosphorylating activity between 40 14%; cerebral cortex were regions maximal activity. Low hexokinases characterized several criteria. Also, using RT‐PCR analysis regulatory previously reported liver. These findings indicate present should facilitate adaptation organ fluctuations blood concentrations, GLUT‐2 same hypothalamic neurons suggests role sensing.