Correlating Physiology with Gene Expression in Striatal Cholinergic Neurones

• DOI: 10.1046/j.1471-4159.2000.740839.x
• Publication Date: 2003-03-12T06:43:45Z
• Authors: Peter J. Richardson; Alistair K. Dixon; Kevin Lee; Matt I. Bell; Peter J. Cox; Richard Williams; Robert D. Pinnock; Tom C. Freeman
• Keywords: Male; Neurons; Cytoplasm; Neurotransmitter Agents; Cytological Techniques; Action Potentials; Gene Expression; In Vitro Techniques; Polymerase Chain Reaction; Corpus Striatum; Choline O-Acetyltransferase; Rats; Receptors, Neurotransmitter; Rats, Sprague-Dawley; 03 medical and health sciences; 0302 clinical medicine; Interneurons; Animals; RNA, Messenger

Abstract: The expression of 34 transmitter‐related genes has been examined in the cholinergic neurones rat striatal brain slices, with aim correlating gene functional activity. mRNAs encoding types I, II/IIA, and III α subunits voltage‐sensitive sodium channels were detected, suggesting presence these three channel. Similarly, all four α‐amino‐3‐hydroxy‐5‐methyl‐isoxazole‐4‐propionate (AMPA)‐type glutamate receptor NR1 NR2A, 2B, 2D NMDA‐type receptors that various combinations mediate cellular response to synaptically released glutamate. Other detected included NK1 NK3 tachykinin receptors, known adenosine GABA‐synthesising enzyme decarboxylase. Subpopulations have identified on basis 5% trkC neurotrophin 12% cells investigated.