Intracellular nickel accumulation by Pseudomonas aeruginosa and its chemical nature

• DOI: 10.1046/j.1472-765x.2001.00878.x
• Publication Date: 2003-03-12T10:10:22Z
• Authors: P. Sar; S.K. Kazy; S.P. Singh
• Keywords: Pseudomonadales

Aims: To investigate intracellular localization of nickel and its chemical nature in Pseudomonas aeruginosa. Methods Results: Transmission electron micrographs Ni‐loaded bacteria exhibited a darkened opaque zone throughout the cell periphery. Energy dispersive X‐ray analysis confirmed deposition metallic nickel. Cell fractionation revealed that 88% accumulated was restricted to periplasm membrane. diffraction patterns ascertained cellular Ni as phosphide (Ni5P4, NiP2 Ni12P5) carbide (Ni3C) crystals. Conclusions: aeruginosa crystal mostly envelope region, indicating predominant role phosphoryl carboxyl/carbonyl groups wall/membrane components cation sequestration. Significance Impact Study: The data contribute significantly better understanding bacteria–metal interaction will be useful developing biotechnological strategies for toxic metal bioremediation.