Salivary Extracellular MicroRNAs for Early Detection and Prognostication of Esophageal Cancer: A Clinical Study

• DOI: 10.1053/j.gastro.2023.06.021
• Publication Date: 2023-07-01T15:00:44Z
• Authors: Kai Li; Yusheng Lin; Yu Zhou; Xiao Xiong; Lu Wang; Junkuo Li; Fuyou Zhou; Yi Guo; Shaobin Chen; Yuping Chen; Hui Tang; Xiaofu Qiu; Songwang Cai; Dianzheng Zhang; Edwin Bremer; Sai-Ching Jim Yeung; Hao Zhang
• Keywords: Cancer Detection

Early detection of esophageal squamous cell carcinoma (ESCC) will facilitate curative treatment. We aimed to establish a microRNA (miRNA) signature derived from salivary extracellular vesicles and particles (EVPs) for early ESCC prognostication.Salivary EVP miRNA expression was profiled in pilot cohort (n = 54) using microarray. Area under the receiver operator characteristic curve (AUROC) least absolute shrinkage selector operation regression analyses were used prioritize miRNAs that discriminated patients with controls. Using quantitative reverse transcription polymerase chain reaction, candidates measured discovery 72) lines. The prediction models biomarkers training 342) validated an internal 207) external 226).The microarray analysis identified 7 distinguishing control subjects. Because 1 not always detectable lines, other 6 formed panel. A this panel accurately all-stage (AUROC 0.968) successfully 2 independent cohorts. Importantly, could distinguish early-stage (stage Ⅰ/Ⅱ) subjects 0.969, sensitivity 92.00%, specificity 89.17%) (sensitivity 90.32%, 91.04%) 91.07%, 88.06%) validation Moreover, prognostic based on established efficiently predicted high-risk cases poor progression-free survival overall survival.The EVP-based 6-miRNA can serve as noninvasive risk stratification ESCC. Chinese Clinical Trial Registry, ChiCTR2000031507.