Enhanced expression of B7-1, B7-2, and intercellular adhesion molecule 1 in sinusoidal endothelial cells by warm ischemia/reperfusion injury in rat liver

• DOI: 10.1053/jhep.2001.27804
• Publication Date: 2002-08-25T03:46:10Z
• Authors: Naosuke Kojima; Mitsuru Sato; Akira Suzuki; Takeya Sato; Shigeru Satoh; Tetsuro Kato; Haruki Senoo
• Keywords: Immunofluorescence

To elucidate a role of costimulatory molecule and cell adhesion in hepatic ischemia/reperfusion injury, we examined an alteration B7-1 (CD80), B7-2 (CD86), intercellular 1 (ICAM-1; CD54) expression the rat liver after warm injury. induce ischemia model, both portal vein artery entering left-lateral median lobes were occluded by clamping for 30 minutes or 60 minutes, then reperfused 24 hours. B7-1, B7-2, ICAM-1 expressions analyzed immunofluorescence staining real-time reverse transcription polymerase chain reaction (RT-PCR). Although at very low levels tissues from normal sham-operated control rats, enhanced protein messenger RNA (mRNA) affected, left ischemia/reperfusion. mRNA constitutively expressed further up-regulated Localization increased proteins, as well von Willebrand factor marker endothelial cells, was confined to sinusoidal cells lobules. Data quantitative RT-PCR analysis revealed that elevated (5.13- 52.9-fold increase, respectively), whereas rather constitutive but (4.24-fold increase). These results suggest play pivotal antigen-presenting expressing and/or might be primary target prevent early rejection inflammatory reactions injury associated with transplantation.