Naked DNA injection for liver metastases treatment in rats

• DOI: 10.1053/jhep.2002.32709
• Publication Date: 2003-02-17T22:18:10Z
• Authors: Patrick Baqué; Valérie Pierrefit...; Adolfo Gavelli; Nicole Brossette; Daniel Benchimol; André Bourgeon; Pascal Staccini; Marie-Christine S...; Bernard Rossi
• Keywords: Cytosine deaminase; Syngenic; Systemic administration

The cytosine deaminase (CD) gene converts the nontoxic prodrug, 5-fluorocytosine (5-FC), into 5-fluorouracil (5-FU). We previously showed that injection of CD-bearing cancer cells followed by 5-FC treatment can act as an autologous tumor vaccine in a syngenic liver metastasis model rats. In present work, we analyzed antitumor efficiency direct intratumoral CD-expressing plasmid. rats bearing microscopic or macroscopic metastases right and left lobes, plasmid was performed lobe tumor, animals. A significant regression DNA-injected observed 5-FC-treated rats, both ( P = .007) advanced < .0001) models. Moreover, this also induced potent distant bystander effect on untreated controlateral tumors extrahepatic metastases, resulting increased survival compared with control animals models .05). conclusion, these data suggest plasmid, associated to administration, constitute powerful innocuous alternative for unresectable from colon carcinoma.