Modulation of paclitaxel resistance by annexin IV in human cancer cell lines

• DOI: 10.1054/bjoc.2000.1311
• Publication Date: 2002-07-26T11:34:15Z
• Authors: E Kyu-Ho Han; S K Tahir; S P Cherian; N Collins; S-C Ng
• Keywords: Annexin A2; Annexin A1

A recurring problem with cancer therapies is the development of drug resistance. While investigating protein profile cells resistant to a novel antimitotic compound (A204197), we discovered an increase in annexin IV expression. When examined expression level paclitaxel-resistant cell line (H460/T800), found that was also overexpressed. Interestingly closely related protein, II, not overexpressed H460/T800 cells. Immunostaining either II or antibody revealed primarily located nucleus Short-term treatment H460 10 nM paclitaxel for up 4 days resulted induction IV, but In addition, there staining starting at day 1. Furthermore, pretreated becoming approximately fivefold more paclitaxel. Transfection cDNA into 293T threefold Thus our results indicate plays role resistance this and it among one earliest proteins induced response cytotoxic stress such as treatment.