Soluble Fas ligand released by colon adenocarcinoma cells induces host lymphocyte apoptosis: an active mode of immune evasion in colon cancer

• DOI: 10.1054/bjoc.2001.2042
• Publication Date: 2009-07-02T16:02:17Z
• Authors: E Song; J Chen; N Ouyang; F Su; M Wang; U Heemann
• Keywords: Jurkat cells; Fas ligand

Expression of membrane-bound Fas ligand (mFasL) on colon cancer cells serves as a potential mechanism to inhibit host immune function by inducing apoptosis lymphocytes. Membrane-bound FasL can be cleaved and released soluble mediator (sFasL), which may spread the induction effect. Our study examined whether adenocarcinoma release sFasL, induce lymphocytes without direct cell-cell contact. In 12 consecutive patients with mFasL was identified in tumours, sFasL measured sera tumour-infiltrating peripheral blood To analyse were primarily cultured; isolated from supernatants, measured, incubated Fas-bearing Jurkat cells, resulting analysed. Serum levels significantly elevated all expression tumour tissues (n = 8). these patients, number apoptotic increased within blood. Furthermore, present corresponding supernatants induced dose-dependent manner. These findings suggest that mFasL-positive thus for evasion.