Cytoskeleton Disorganization during Apoptosis Induced by Curcumin in A549 Lung Adenocarcinoma Cells

• DOI: 10.1055/s-0029-1185399
• Publication Date: 2009-03-06T04:43:55Z
• Authors: Qingyong Chen; Guohua Lu; Yanyi Wang; Yingke Xu; Ying Zheng; Li Yan; Zhongyong Jiang; Ling Yang; Jianwei Zhan; Yuquan Wu; Jianying Zhou
• Keywords: Cytochalasin D; Stress fiber; Cytochalasin; Viability assay; Actin remodeling; Phalloidin

Several studies have shown that curcumin can induce apoptosis and inhibit growth in human A549 lung adenocarcinoma cells. However, the mechanism is not completely understood yet. In present study, we investigated vitro effect of on cell viability, disorganization actin cytoskeleton Our results showed significantly inhibited viability cells a dose- time-dependent manner by induced apoptosis. The apoptotic process was associated with architecture microfilaments decrease levels F-actin. DMSO-treated control exhibited well-defined F-actin network mainly organized into stress fibers. fibers treated or positive drug cytochalasin B were disorganized, disassembled, disrupted, however, could be prevented phalloidin, an stabilizing compound. Thus, these demonstrated filament might play central role curcumin-induced