Development of a Fast and Convenient Method for the Isolation of Triterpene Saponins fromActaea racemosaby High-speed Countercurrent Chromatography Coupled with Evaporative Light Scattering Detection

• DOI: 10.1055/s-0029-1186236
• Publication Date: 2009-10-22T00:39:10Z
• Authors: Serhat Cicek; Stefan Schwaiger; Ernst Ellmerer; Hermann Stuppner
• Keywords: Aglycone; Fraction (chemistry)

In the present work, a fast and simple method for separation purification of triterpene saponins from Actaea racemosa was successfully established. Accelerated solvent extraction used defatting extracting subaerial parts, giving enriched crude extract. Size exclusion chromatography to separate actein 23-epi-26-deoxyactein other triterpenoids, which were collected in third fraction. This most complex fraction applied high-speed countercurrent chromatography, well-established technique saponins. Separation parameters first optimized on an analytical level, using hyphenated HSCCC-ELSD setup, before system scaled up preparative size. The resulting two-phase system, consisting N-hexane-acetone-ethyl acetate-2-propanol-ethanol-water (3.5 : 1 2 0.5 2, v/v/v/v/v/v), enabled isolation 23-O-acetylshengmanol-3-O- beta-D-xylopyranoside (17.4 mg), cimiracemoside D (19.5 25-O-acetylcimigenol-3-O-beta-D-xylopyranoside (7.1 mg) aglycone cimigenol (5.9 mg). Purity isolated substances 96.8 %, 96.2 97.9 98.4 respectively. same suitable 23-epi-26-deoxyactein, with purities 97.0 % 98.3 %.