A reliable and efficient protocol for inducing hairy roots in Agastache foeniculum

• DOI: 10.1055/s-0034-1382518
• Publication Date: 2014-07-14T22:35:08Z
• Authors: E Nourozi; B Hosseini; A Hassani; S Rostami; Z Moghaddamfar
• Keywords: Hairy Root Culture; Foeniculum; Rosmarinic acid

Hairy root culture system is a valuable tool to study the characteristics of gene expression, function, biology, biochemical properties and biosynthesis pathways secondary metabolites. In present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains A. rhizogenes, (A4, A7 9435), used for induction four various explants (hypocotyl, cotyledon, 1 – month old leaf 5 leaf) hyssop. The highest frequency transformation was achieved using A4 strain (51.1%). Polymerase chain reaction (PCR) analysis with specific primers rol B confirmed putative transgenic roots. separate experiment obtained from inoculated leaves strain, grown liquid medium effects different concentrations salicylic acid (0.0, 0.01, 0.1 mM/l) chitosan (0, 50, 100 150 mg/l) as elicitors sucrose (20, 30, 40 50 g/l) on growth evaluated. results showed that, 30 g/l mg/l increased biomass application reduced compared control. HPLC revealed that Rosmarinic levels about fourfold higher non transformed plants.