Radiolabeling of Fibrinogen Using the Iodogen Technique

• DOI: 10.1055/s-0038-1653425
• Publication Date: 2018-07-05T22:38:01Z
• Authors: Linda C Knight; Andrei Z Budzynski; Stephanie A Olexa
• Keywords: Iodine monochloride; Oxidizing agent

The properties of human fibrinogen labeled with 125-Iodine using Iodogen (1, 3, 4, 6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril) as an oxidizing agent were compared those iodine monochloride counterpart. It was found that thrombin clottability, binding to staphylococci, the relative specific radioactivity A B beta, and gamma chains in vivo clearance from plasma rabbits same these two preparations. Labeling efficiency higher when iodogen used. is concluded radioiodine technique suitable for studies vitro vivo.