Assay of Human Tissue-Type Plasminogen Activator (t-PA) with an Enzyme-Linked Immunosorbent Assay (ELISA) Based on Three Murine Monoclonal Antibodies to t-PA

• DOI: 10.1055/s-0038-1660097
• Publication Date: 2018-07-19T18:36:21Z
• Authors: Paul Holvoet; Hugo Cleemput; Désiré Collen
• Keywords: Polyclonal antibodies; Microtiter plate; Assay sensitivity; T-plasminogen activator

An enzyme-linked immunosorbent assay (ELISA) for the measurement of human tissue-type plasminogen activator (t-PA) was developed. Microtiter plates were coated with a mixture two monoclonal antibodies and bound t-PA quantitated third antibody linked to peroxidase. The lower limit sensitivity 0.2 ng per ml. concentration antigen in citrated plasma subjects found be 3.4 +/- 0.8 ng/ml. had good reproducibility values 3.8, 6.5 4.9 percent respectively intra-, inter-assay inter-dilution variation coefficients. results ELISA on samples from patients during thrombolytic therapy correlated very well, over wide range, those obtained previously described two-site immuno-radiometric (r = 0.96). This constitutes stable reproducible set reagents biological fluids, avoiding disadvantages use radioisotopes polyclonal antibodies.