A Computer Assisted Method to Obtain the Prothrombin Activation Velocity in Whole Plasma Independent of Thrombin Decay Processes

• DOI: 10.1055/s-0038-1661594
• Publication Date: 2018-07-13T22:35:03Z
• Authors: H C Hemker; G M Willems; S Béguin
• Keywords: Thrombin Generation; Alpha (finance)

A method is described that, on the basis of time course amidolytic activity after triggering thrombin generation in normal plasma, allows calculation velocity prothrombin conversion independent inactivating processes. It shown how reaction constants for alpha 2M-dependent and 2M-independent inactivation processes can be obtained a sample whole plasma. The verified by demonstrating that experimentally observed courses residual 2M-thrombin complex coincide with those calculated from activity, showing plasma without 2-macroglobulin or AT III adequately if 2M III-dependent breakdown are taken zero calculations. appears thrombin, endogenously generated about half as fast exogenous added to computer program presented carries out relevant