Increased Neutrophil Activation and Plasma DNA Levels in Patients with Pre-Eclampsia

• DOI: 10.1055/s-0038-1675788
• Publication Date: 2018-11-19T23:47:56Z
• Authors: Yae Hu; Hui Li; Ruhong Yan; Can Wang; Yun Wang; Ce Zhang; Meng Liu; Tiantian Zhou; Weipei Zhu; Hong Zhang; Ningzheng Dong; Qingyu Wu
• Keywords: Neutrophil Extracellular Traps

Abstract Pre-eclampsia (PE) is a chronic inflammatory disease in pregnancy, which associated with enhanced blood coagulation and high thrombotic risk. To date, the mechanisms underlying such an association are not fully understood. Previous studies reported levels of plasma deoxyribonucleic acid (DNA) PE women, but cellular source circulation DNA remains unknown. In this study, we tested hypothesis that activated neutrophils undergoing cell death, also called NETosis, may be responsible for elevated women. We analysed samples from non-pregnant, normal pregnant women found double-stranded DNA, myeloperoxidase (an abundant neutrophil granular enzyme) histones (the major nucleosome proteins) PE-derived samples, indicating increased NETosis maternal circulation. The positively correlated When isolated individuals were incubated plasma, NETosis-stimulating activity was detected. Further experiments showed endothelial micro-particles, soluble proteins, primarily These results indicate circulating micro-particles damaged endothelium potent stimulator activation Given pro-coagulant pro-thrombotic nature nuclear contents neutrophils, systemic represent important mechanism hyper-coagulability risk PE.