Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

• DOI: 10.1063/1.3671399
• Publication Date: 2012-01-06T16:48:08Z
• Authors: Yung-Shin Sun; Shih-Wei Peng; Keng-Hui Lin; Ji-Yen Cheng
• Keywords: Gelatin; 3D cell culture; Morphology

In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells foam scaffolds which resemble vivo pulmonary alveoli may give more insight on cellular behaviors vivo. consists ordered arrays uniform spherical pores gelatin. We found that cell morphology was different from 2D substrate. Next, applied direct current electric field (EF) 338 mV/mm through for study cells' migration within. measured directedness and speed lines, CL1-0, CL1-5, A549, compared those examined gelatin-coated bare substrates. direction is same all conditions but there are clear differences morphology, directedness, under EF. Our results demonstrate EF environments possibly due and/or substrate stiffness.