Quantum dot-based microfluidic biosensor for cancer detection

• DOI: 10.1063/1.4921203
• Publication Date: 2015-05-15T17:00:37Z
• Authors: Aditya Sharma Ghrera; Chandra Mouli Pandey; Md. Azahar Ali; Bansi Dhar Malhotra
• Keywords: Polydimethylsiloxane; Microchannel; Nanopore

We report results of the studies relating to fabrication an impedimetric microfluidic–based nucleic acid sensor for quantification DNA sequences specific chronic myelogenous leukemia (CML). The chip is prepared by patterning indium–tin–oxide (ITO) coated glass substrate via wet chemical etching method followed sealing with polydimethylsiloxane (PDMS) microchannel fluid control. fabricated microfluidic comprising a patterned ITO modified depositing cadmium selenide quantum dots (QCdSe) Langmuir–Blodgett technique. Further, QCdSe surface has been functionalized probe CML detection. integrated miniaturized system used monitor target complementary concentration measuring interfacial charge transfer resistance hybridization. presence in buffer solution significantly decreased electro-conductivity interface due barrier transport redox ions. biosensor exhibits improved linearity range 10−15 M 10−11 M.