Correlation between cell migration and reactive oxygen species under electric field stimulation

• DOI: 10.1063/1.4932662
• Publication Date: 2015-10-06T17:00:31Z
• Authors: Shang-Ying Wu; Hsien-San Hou; Yung-Shin Sun; Ji-Yen Cheng; Kai-Yin Lo
• Keywords: Multicellular organism

Cell migration is an essential process involved in the development and maintenance of multicellular organisms. Electric fields (EFs) are one many physical chemical factors known to affect cell migration, a phenomenon termed electrotaxis or galvanotaxis. In this paper, microfluidics chip was developed study cells under different electrical stimuli. This capable providing four strengths EFs combination with two chemicals via simple set agar salt bridges Ag/AgCl electrodes. NIH 3T3 fibroblasts were seeded inside their reactive oxygen species (ROS) production response EF presence β-lapachone. We found that both β-lapachone level increased rate ROS EF-strength-dependent manner. A strong linear correlation between amount intracellular suggests intermediate product by which enhance migration. Moreover, anti-oxidant, α-tocopherol, quench ROS, resulting decrease rate.