High-precision high-speed nanopore ping-pong control system based on field programmable gate array

• DOI: 10.1063/5.0213543
• Publication Date: 2024-07-17T12:37:59Z
• Authors: Zhuang Mi; Xiaoyu Chen; Xinjia Zhao; Haitao Tang; Wenyu Wang; Xinyan Shan; Xinghua Lu
• Keywords: Ping pong; Ping (video games); Nanopore; Gate array

"Molecular ping-pong," emerging as a control strategy in solid-state nanopore technology, presents highly promising approach for repetitive measurements of single biomolecules, such DNA. This paper introduces high-precision, high-speed molecular ping-pong system consisting home-built trans-impedance amplifier (TIA), based on Field Programmable Gate Array (FPGA), and LabVIEW program operating the host personal computer. Through feedback compensation post-stage boosting, TIA achieves high bandwidth about 200 kHz with gain 100 MΩ, along low input-referred current noise 1.6 × 10-4 pA2/Hz at 1 1.1 10-3 kHz. The FPGA-based demonstrates minimum overall response time (tdelay) 6.5 μs from analog input signal trigger to subsequent reversal output drive voltage signal, precision μs. Additionally, has been developed facilitate rapid data exchange communication FPGA program, enabling real-time monitoring, parameter adjustment, storage. Successful recapture individual DNA molecules various tdelay, resulting an improvement capture rate by up 2 orders magnitude, demonstrated. With unprecedented efficiency, this provides robust technical support opens novel research avenues single-molecule sensing manipulation.