Rapid wavelength interval selection technology for multi-channel intensity-interrogation surface plasmon resonance imaging sensing

• DOI: 10.1063/5.0236924
• Publication Date: 2024-10-14T10:18:12Z
• Authors: Xiaorui Liu; Songfeng Huang; Jiali Tai; Hailong Ni; Xiaoqi Dai; Xueliang Wang; Jiajie Chen; Ho-Pui Ho; Ping Qiu; Yuye Wang; Yonghong Shao
• Keywords: Interrogation; Localized surface plasmon

Intensity-interrogation surface plasmon resonance (I-SPR) provides the fastest imaging speed among various SPR techniques, including wavelength, phase, and angle-based detection. It also integrates easily with other devices has substantial practical application potential. However, I-SPR's reliance on direct detection of reflected light intensity renders it sensitive to source fluctuations detector dark noise, its dynamic range is relatively limited. To address these challenges, we have developed a multi-channel I-SPR sensing platform featuring rapid wavelength interval selection technology. This system optimizes according refractive indices different samples, mitigating issues related inconsistent signals from biomolecules. Our improved technology enables high-throughput biomolecular index size 2.035 × 10−2 for monitoring, reaching leading role existing technologies. represents widest linear date. Experimental results protein binding kinetic constants KD are consistent those obtained commercially available instruments. We believe that this study expected accelerate development toward broader biological applications.