Interspecific introgression of male sterility from tetraploid oilseed Brassica napus to diploid vegetable B. rapa through hybridisation and backcrossing

• DOI: 10.1071/cp13103
• Publication Date: 2013-10-04T05:34:16Z
• Authors: Zhengjie Wan; Yuanbao Tan; Minhui Shi; Yuejin Xu; Nader Aryamanesh; Guijun Yan
• Keywords: Introgression; Brassica rapa

Interspecific F1 hybrids were obtained from a cross between male sterile Brassica napus (2n = 4x 38, AA (20) and CC (18) genomes) an inbreeding line B. rapa (Purple Cai-Tai inbred 9418, 2n 2x 20, genome) to introgress sterility tetraploid into diploid through backcrossing. The morphological characteristics of plants more like the female parent segregated considerably in BC1 when backcrossed recurrent Purple Cai-Tai. progeny became stable similar by BC4. Most C genome chromosomes found be eliminated, based on cytogenetic analysis. majority eliminated at very early backcross stages, with only 20–26 plants, some gradually increased generations. BC4 generally exactly 20 chromosomes. Analysis AFLP indicated that 49.5–68.7% total bands specific, ~12% retained genetic materials controlling introgressed successfully even though most chromosomes/genetic during process.