Expression of a Chicken Ovalbumin Gene in Three Lucerne Cultivars

• DOI: 10.1071/pp9910495
• Publication Date: 2006-01-04T01:20:09Z
• Authors: HE Schroeder; MRI Khan; WR Knibb; D Spencer; TJV Higgins
• Keywords: Callus; Cauliflower mosaic virus; Chimeric gene

Routine procedures have been developed for the transformation of lucerne (Medicago sativa cv. Rangelander) with foreign genes using Agrobacterium tumefaciens binary vector system and regeneration transgenic plants from tissue culture, via somatic embryogenesis. Lucerne was carried out a gene encoding neomycin phosphotransferase (npt), which conferred resistance to antibiotic kanamycin, together cDNA clone chicken ovalbumin modified expression in plant cells. The protein coding sequence combined cauliflower mosaic virus 35S promoter nopaline synthase 3' flanking make chimeric gene. A DNA construct containing both these transferred lucerne, detected leaves regenerated immunoblots. Pulse-chase labelling experiments analysis top bottom transformed indicated that ovalbumin, once formed, stable lucerne. wide variation level frequently observed multiple embryos on single callus. This correlated changes restriction enzyme digestion pattern plants. These results indicate each callus may arisen more than one event. An alternative interpretation is cell but during proliferation some cells undergone rearrangement prior Transformation were also two Australian commercial cultivars Although frequency recovery lower Rangelander, protocols open way relatively rapid