Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments

• DOI: 10.1071/rd09197
• Publication Date: 2010-04-29T01:25:20Z
• Authors: Álvaro E. Domíngu...; María R. Fernánde...; Alfonso Bisbal; José Luis Ros-San...; Manuel Ramón; Manuel Carmona; Felipe Martínez-P...; J. Julián Garde
• Keywords: Trolox; Malondialdehyde

Antioxidants could improve sperm media, extending the viability of spermatozoa and protecting their DNA. The protective ability lipoic acid, melatonin, Trolox crocin was tested on red deer incubated at 37°C. Cryopreserved were thawed with 1 mM or 0.1 each antioxidant, without oxidative stress (100 μM Fe2+). Motility (CASA), viability, mitochondrial membrane potential acrosomal status assessed. Lipoperoxidation (malondialdehyde production), intracellular reactive oxygen species (ROS) DNA (TUNEL) checked 4 h. Incubation alone increased ROS decreased motility. Oxidative intensified these effects, increasing lipoperoxidation damage. Lipoic acid had little effect, whereas melatonin showed limited protection. lowered both in oxidised non-oxidised samples. In samples, prevented damage, ameliorated Crocin similar results to Trolox, but noticeably stimulated motility no effect lipoperoxidation. a second experiment, broader range concentrations tested, confirming effects (positive noticeable 0.5–0.75 mM), showing an increase 2 mM. Melatonin increasingly effective 2.5 5 (ROS, status). seems promising new its particular physiology must be further studied, especially consequences stimulation might useful relatively high concentrations, compared Trolox.