Effect of docosahexanoic acid on quality of frozen–thawed bull semen in BioXcell extender

• DOI: 10.1071/rd15089
• Publication Date: 2015-09-08T04:29:39Z
• Authors: Asmatullah Kaka; Wahid Haron; Rosnina Yusoff; Nurhusien Yimer; A. M. Khumran; Kazhal Sarsaifi; Atique Ahmed Behan; Ubedullah Kaka; Akeel Ahmed Memon; Mahdi Ebrahimi
• Keywords: Semen quality

This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on quality frozen-thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight each bull). Ejaculates with motility ≥70% and normal morphology ≥80% extended into which 0 (control), 3, 5, 10 or 15ngmL-1 DHA added. The supplemented semen samples incubated at 37°C for 15min uptake by spermatozoa. Later, cooled 2h 5°C packaged 0.25-mL straws, frozen liquid nitrogen 24h subsequently thawed evaluation. Results are presented as percentages ± s.e.m. Supplementation 3ngmL-1 significantly improved sperm functional parameters including motility, morphology, viability, acrosome integrity membrane when compared other groups control. Lipid peroxidation increased incorporation increased. In conclusion, concentration resulted superior spermatozoa is suggested optimum level be added extender.