Cryopreservation of macropodid spermatozoa: new insights from the cryomicroscope

• DOI: 10.1071/rd99076
• Publication Date: 2003-11-17T03:41:56Z
• Authors: W. V. Holt; L. M. Penfold; S. D. Johnston; P. Temple-Smith; J. Shaw; C. McCallum; W. Lindemans; D. Blyde
• Keywords: Cryoprotectant

This study examined the effects of cooling and cryopreservation upon macropod spermatozoa (eastern grey kangaroo, Macropus giganteus red-necked wallaby, rufogriseus). Sperm survival during after freezing to –30˚C or –70˚C in minimum essential medium (MEM) + 5, 10, 20 30% (v/v) glycerol, MEM 10 20% ethylene glycol containing a mixture 7.5% glycerol 10% dimethylsulphoxide was by cryomicroscopy. The MEM/glycerol mixtures permitted better post-thaw sperm recovery than other cryoprotectants. After at 10˚C min –1 then rewarming 20˚C , flagellar activity resumed more 50% when temperature increased into range 5–10˚C. However, as increased, 20–25˚C, motility declined rapidly so that less 5% motile cells were seen 35˚C. Spermatozoa without cryoprotectant also cryomicroscopy evaluate changes configuration, swimming behaviour viability from 35˚C approximately –7˚C, Cooling 35 28˚C induced kangaroo exhibit rigid principal-piece bending non-linear motility, which reversed further their normal linear movement. Rewarming 20–30˚C, but this effect warming. Although wallaby showed these effects, they exhibited tendency form rosette-like clusters rewarming, especially reached 14˚C. end-pieces became anteriorly reflected, producing hook-like conformations, interlinked.