173 EFFECTS OF GLUCOSE METABOLISM DURING IN VITRO MATURATION ON CYTOPLASMIC MATURATION OF GOAT OOCYTES

• DOI: 10.1071/rdv26n1ab173
• Publication Date: 2013-12-05T01:20:15Z
• Authors: J.-H. Tan; Y.-B. Wang; H.-L. Xie; Q. Li; X.-Y. Liu; P. Zhou; M.-J. Luo
• Keywords: In vitro maturation; Sodium pyruvate; Carbohydrate Metabolism; Ionomycin

It is well known that oocyte maturation consists of 2 processes: nuclear and cytoplasmic maturation. Nuclear refers to resumption the first meiosis extrusion polar body (PB1), manifested as acquisition ability complete pre-implantation development. Although it recognised energy supply essential for there have been many reports on effect glucose metabolism maturation, studies ooplasmic are limited. In present study, goat oocytes recovered from slaughterhouse ovaries were cultured 24 h in a simplified CR1 (sCR1) medium (NaCl, KCl, NaHCO3, CaCl2, BSA, eCG) supplemented with (10 mM) and/or lactate (3.5 presence or absence pentose phosphate pathway (PPP) inhibitor dehydroepiandrosterone (DHEA, 100 μM) glycolysis iodoacetate (1 μM). At end culture, PB1 either activated by treatment ionomycin plus 6-DMAP observe embryo development, assayed total glutathione concentrations (GSX) reduced (GSH)/oxidized (GSSG) ratios. Embryos 9 days CR1aa calcium lactate, sodium pyruvate, glutamine, EAA, NEAA, FCS) at 38.5°C under 5% CO2 humidified air. inhibitors, rates 82, 65, 76%, blastocyst 7, 0, 7%, obtained, respectively, after matured sCR1 glucose, both. When containing DHEA iodoacetate, 69 67%, no produced case. However, whereas 12% morulae, morulae observed iodoacetate. Furthermore, GSX (pmol/oocyte) 8.5, 6.5, 7.2, GSH/GSSG ratios 1.8, 0.3, 0.5, without inhibitors 300 μM 3 The difference concentration was statistically significant (P < 0.05; one-way ANOVA) between conclusion, using culture system (sCR1 3.5 mM lactate) sustained but did not support we studied PPP oocytes. results suggest both oocytes, promote increasing synthesis reduction. This study supported grants National Basic Research Program China (Nos. 2012CB944403 2014CB138503) Natural Science Foundation 31272444 30972096).