186 SUPPLEMENTATION WITH LOW DOSES OF DIMETHYL SULFOXIDE DURING IN VITRO MATURATION RESULTS IN IMPROVED IN VITRO EMBRYO PRODUCTION IN CATTLE

• DOI: 10.1071/rdv29n1ab186
• Publication Date: 2016-12-01T23:35:05Z
• Authors: A. E. Ynsaurralde; M. Suvá; R. Bevacqua; S. Munilla; C. Luchetti; D. F. Salamone
• Keywords: In vitro maturation; Cysteamine

Oocyte in vitro maturation (IVM) is crucial for subsequent embryo production. It involves acquisition of competence fertilization and development. Therefore, its optimization could have a direct impact on Dimethyl sulfoxide (DMSO) commonly used as solvent or vehicle, but also increases the membrane permeability behaves scavenger cytotoxic free radicals. The aim this study was to evaluate effect DMSO supplementation during bovine oocyte development determine optimal usage dose with no toxic effect. To aim, cumulus-oocyte complexes were collected from slaughterhouse ovaries IVM TCM 199 containing 10% fetal serum, 10 µg mL−1 FSH, 0.3 mM sodium pyruvate, 100 cysteamine, 2% antibiotic-antimycotic. oocytes incubated 24 h at 6.5% CO2 humidified air 38.5°C. For Experiment 1, medium supplemented concentrations 0, 0.1, 0.5, (vol/vol) (n = 241, 195, 42, 192, 172 oocytes) rate determined by presence first polar body. 2, 0.25, 0.75, 446, 322, 65, 194, 77, 250, 39 cleavage blastocyst rates establish dose. In performed according Brackett Oliphant (1975), 16 × 106 spermatozoa/mL 5 h. Afterwards, presumptive zygotes cultured SOF 7 days 38.5°C 5% O2. Cleavage Days 2 7, respectively. Results statistically analysed using Fisher’s exact test GraphPad Prism software (GraphPad Software Inc., La Jolla, CA, USA). Also, percentage adjusted concentration R quadratic regression model. optimum calculating maximum estimated predictive equation. resulted significantly lower body extrusion (0% 74%a, 0.1% 73%a, 0.5% 83%a, 1% 66%a, 8%b; different letters indicate statistical differences) 75%a, 77%a, 0.25% 80%a, 79%a, 0.75% 78%a, 3%b) than other treatments. Furthermore, production higher 0.25 groups 26%b, 37%ab, 40%a, 41%a, 34%ab, 23%b, 0%c). equation results that blastocysts obtained 0.458% DMSO. conclusion, had positive Further studies will be carried out elucidate mechanism action.