Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome

• DOI: 10.1073/pnas.012585199
• Publication Date: 2002-07-26T14:36:44Z
• Authors: Minoru Funakoshi; Toru Sasaki; Takeharu Nishimoto; Hideki Kobayashi
• Keywords: Budding yeast; C-terminus

Dsk2p from Saccharomyces cerevisiae belongs to the class of proteins that contain a ubiquitin-like (UbL) domain at N terminus together with ubiquitin-associated (UBA) C terminus. We show here C-terminal UBA binds K48-linked polyubiquitin chains, and N-terminal UbL interacts proteasome. Overexpression caused accumulation large amounts polyubiquitin, extragenic suppressors Dsk2p-mediated lethality proved be temperature-sensitive mutations in two proteasome subunits, rpn1 pre2. ubiquitin-dependent degradation was impaired by disruption DSK2 gene. These results indicate is polyubiquitin-binding protein also discuss possible role adaptor function via its domains ubiquitin-proteasome pathway.