Evolution of the MAT locus and its Ho endonuclease in yeast species

• DOI: 10.1073/pnas.0304170101
• Publication Date: 2004-02-10T18:32:51Z
• Authors: Geraldine Butler; Claire Kenny; Ailís Fagan; Cornelia Kurischko; Claude Gaillardin; Kenneth H. Wolfe
• Keywords: 570; 0303 health sciences; Saccharomyces cerevisiae Proteins; Molecular Sequence Data; SILENT HMR/HLM CASSETTE; High Mobility Group Proteins; [SDV.BC]Life Sciences [q-bio]/Cellular Biology; Saccharomyces cerevisiae; YEAST;MATING TYPE SWITCHING;HO ENDONUCLEASE;SILENT HMR/HLM CASSETTE; MATING TYPE SWITCHING; Evolution, Molecular; 03 medical and health sciences; YEAST; HO ENDONUCLEASE; Deoxyribonucleases, Type II Site-Specific; levure

The genetics of the mating-type ( MAT ) locus have been studied extensively in Saccharomyces cerevisiae , but relatively little is known about how this complex system evolved. We compared the organization of MAT and mating-type-like ( MTL ) loci in nine species spanning the hemiascomycete phylogenetic tree. We inferred that the system evolved in a two-step process in which silent HMR / HML cassettes appeared, followed by acquisition of the Ho endonuclease from a mobile genetic element. Ho-mediated switching between an active MAT locus and silent cassettes exists only in the Saccharomyces sensu stricto group and their closest relatives: Candida glabrata, Kluyveromyces delphensis , and Saccharomyces castellii . We identified C. glabrata MTL1 as the ortholog of the MAT locus of K. delphensis and show that switching between C. glabrata MTL1 a and MTL1 α genotypes occurs in vivo . The more distantly related species Kluyveromyces lactis has silent cassettes but switches mating type without the aid of Ho endonuclease. Very distantly related species such as Candida albicans and Yarrowia lipolytica do not have silent cassettes. In Pichia angusta , a homothallic species, we found MAT α 2, MAT α 1 , and MAT a1 genes adjacent to each other on the same chromosome. Although some continuity in the chromosomal location of the MAT locus can be traced throughout hemiascomycete evolution and even to Neurospora , the gene content of the locus has changed with the loss of an HMG domain gene ( MAT a2) from the MAT a idiomorph shortly after HO was recruited.