NADH augments blood flow in physiologically activated retina and visual cortex
Male
0301 basic medicine
Retinal Vessels
NAD
Rats
Rats, Sprague-Dawley
03 medical and health sciences
NG-Nitroarginine Methyl Ester
0302 clinical medicine
Pyruvic Acid
Animals
Lactic Acid
Energy Metabolism
Visual Cortex
DOI:
10.1073/pnas.0307458100
Publication Date:
2004-01-13T18:28:06Z
AUTHORS (3)
ABSTRACT
The mechanism(s) that increase retinal and visual cortex blood flows in response to visual stimulation are poorly understood. We tested the hypothesis that increased transfer of electrons and protons from glucose to cytosolic free NAD
+
, reducing it to NADH, evoked by increased energy metabolism, fuels redox-signaling pathways that augment flow. The near-equilibrium between free cytosolic NADH/NAD
+
and lactate/pyruvate ratios established by lactate dehydrogenase predicts that transfer of additional electrons and protons from injected lactate to NAD
+
will augment the elevated blood flows in stimulated retina and cortex, whereas transfer of electrons and protons from NADH to injected pyruvate will attenuate the elevated flows. These predictions were tested and confirmed in rats. Increased flows evoked by stimulation also were prevented by inhibition of nitric oxide synthase. These findings support an important role for cytosolic free NADH in fueling a signaling cascade that increases
•
NO production, which augments blood flow in photostimulated retina and visual cortex.
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