Gene expression analysis in schizophrenia: Reproducible up-regulation of several members of the apolipoprotein L family located in a high-susceptibility locus for schizophrenia on chromosome 22

• DOI: 10.1073/pnas.032069099
• Publication Date: 2002-07-26T14:37:36Z
• Authors: Michael L. Mimmack; Margaret Ryan; Hajime Baba; Juani Navarro-Ruiz; Shuji Iritani; Richard L. M. Faull; Peter J. McKenna; Peter B. Jones; Heii Arai; Michael Starkey; Piers C. Emson; Sabine Bahn
• Keywords: 0301 basic medicine; Chromosomes, Human, Pair 22; Gene Expression Profiling; Chromosome Mapping; Reproducibility of Results; Apolipoprotein L1; Up-Regulation; 03 medical and health sciences; Apolipoproteins; 0302 clinical medicine; Gene Expression Regulation; Schizophrenia; Humans; Genetic Predisposition to Disease; Lipoproteins, HDL

We screened a custom-made candidate gene cDNA array comprising 300 genes. Genes chosen have either been implicated in schizophrenia, make conceptual sense the light of current understanding disease, or are located on high-susceptibility chromosome locations. The screen using prefrontal cortex tissue from 10 schizophrenia and control brains revealed robust up-regulation apolipoprotein L1 (apo L1) by 2.6-fold. finding was cross-validated blinded quantitative PCR study Stanley Foundation brain collection, Bethesda, MD. This collection consists 15 bipolar disorder, major depression, individuals, all 60 being well-matched conventional parameters, with antipsychotic drug exposure disorder groups. Significant apo expression confirmed. Using PCR, profiles other members L family L2–L6) were investigated, showing that L2 L4 highly significantly up-regulated schizophrenia. Results then confirmed an independent set 20 Japan New Zealand. Apo proteins belong to group high density lipoproteins, six genes close proximity each 22q12, locus for region associated velocardiofacial syndrome includes symptoms