Purification and architecture of the ubiquitous Wave complex

• DOI: 10.1073/pnas.0400628101
• Publication Date: 2004-03-20T01:13:08Z
• Authors: Alexis Gautreau; Hsin-yi H. Ho; Jiaxu Li; Hanno Steen; Steven P. Gygi; Marc W. Kirschner
• Keywords: Nap; HeLa

The Wave proteins are major activators of the Arp2/3 complex. ubiquitous Wave-2 is required for actin polymerization at leading edge migrating cells. Here we purify from HeLa Five proteins, Sra, Nap, Wave-2, Abi, and Hspc, copurified, indicating that they form a tight These only present in complexed form, with exception which displays free pool. We reconstitute complex by cotranslating vitro five subunits use this system together specific immunoprecipitations to study molecular architecture organized around core Nap Abi. Sra peripheral subunit recruited on side, whereas Hspc Abi side core.