Effects of cigarette smoke on the human airway epithelial cell transcriptome

• DOI: 10.1073/pnas.0401422101
• Publication Date: 2004-06-22T00:16:55Z
• Authors: Avrum Spira; Jennifer Beane; Vishal Shah; Gang Liu; Frank Schembri; Xuemei Yang; John Palma; Jerome S. Brody
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Transcription, Genetic; Gene Expression Profiling; Smoking; Humans; Bronchi; Epithelial Cells; Smoking Cessation; 3. Good health

Cigarette smoke is the major cause of lung cancer, leading cancer death, and chronic obstructive pulmonary disease, fourth death in United States. Using high-density gene expression arrays, we describe genes that are normally expressed a subset human airway epithelial cells obtained at bronchoscopy (the transcriptome), define how cigarette smoking alters transcriptome, detail effects variables, such as cumulative exposure, age, sex, race, on smoke-induced changes expression. We also determine which not reversible when discontinued. The persistent altered former smokers may explain risk these individuals have for developing long after they discontinued smoking. use profiling to explore normal biology specific within complex organ across broad spectrum healthy irreversible genetic has been previously reported.