Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin

• DOI: 10.1073/pnas.0403608101
• Publication Date: 2004-09-08T01:33:01Z
• Authors: Vishwanath Ramach...; Marcie Williams; Tadayuki Yago; David W. Schmidtke; Rodger P. McEver
• Keywords: P-Selectin; 0303 health sciences; 03 medical and health sciences; Time Factors; Neutrophils; Cricetinae; Cell Membrane; Animals; Humans; Leukocyte Rolling; 3. Good health

Leukocytes rolling on selectins extrude thin membrane tethers that might stabilize velocities despite marked alterations in wall shear stress. To test this hypothesis, we used differential interference contrast videomicroscopy to visualize formation and breakage of as neutrophils rolled P-selectin under flow. Neutrophils rapidly increased tether number stress rose decreased declined. Membrane invariably accompanied slower, more uniform steps translated into lower mean variances velocity. Unexpectedly, neutrophils, but not fixed cells or microspheres bearing selectin ligands, progressively slowly uniformly over time. Scanning electron microscopy revealed developed larger, complex structures they for longer periods. These data provide evidence selectin-mediated by adjusting response changes Gradual remodeling architecture may further reduce velocities, facilitating integrin-dependent deceleration arrest inflamed vascular surfaces.