A totally synthetic vaccine of generic structure that targets Toll-like receptor 2 on dendritic cells and promotes antibody or cytotoxic T cell responses

• DOI: 10.1073/pnas.0406740101
• Publication Date: 2004-10-16T00:23:41Z
• Authors: David C. Jackson; Yuk Fai Lau; Thuy Le; Andreas Suhrbier; Georgia Deliyannis; Christina Cheers; Corey Smith; Weiguang Zeng; Lorena E. Brown
• Keywords: Male; Mice, Inbred BALB C; Vaccines, Synthetic; Membrane Glycoproteins; Molecular Sequence Data; Toll-Like Receptors; Receptors, Cell Surface; Dendritic Cells; Toll-Like Receptor 2; Cell Line; 3. Good health; Mice, Inbred C57BL; Mice; 03 medical and health sciences; 0302 clinical medicine; Antibody Formation; Animals; Humans; Female; Amino Acid Sequence; T-Lymphocytes, Cytotoxic

A simple generic peptide-based vaccine structure that targets Toll-like receptor 2-expressing dendritic cells and causes their activation is described. The vaccines are totally synthetic, serve as own adjuvant, composed of ( i ) a single helper T cell epitope, ii target epitope either recognized by CD8 + or B cells, iii 2-targeting lipid moiety, S -[2,3-bis(palmitoyloxy)propyl]cysteine, situated between the peptide epitopes to form branched configuration. different examined were from influenza virus, intracellular bacterium Listeria monocytogenes , ovalbumin model tumor antigen. Vaccines containing gastrin luteinizing hormone-releasing hormone also for ability elicit antibody against parent hormones. Each was capable inducing antibody-mediated immune responses. lipidated vaccines, but not nonlipidated able mediate protection viral bacterial infection prophylactic therapeutic anticancer activity. two hormone-based induced high titers, which in case resulted abrogation reproductive function. These results highlight utility simple, epitope-based vaccines.