A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid inNilaparvata lugens(brown planthopper)

• DOI: 10.1073/pnas.0502901102
• Publication Date: 2005-06-04T00:24:58Z
• Authors: Zewen Liu; Martin S. Williamson; Stuart J. Lansdell; Ian Denholm; Zhaojun Han; Neil S. Millar
• Keywords: Brown planthopper; Neonicotinoid; Delphacidae

Neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor (nAChR) agonists with potent insecticidal activity. Since its introduction in the early 1990s, imidacloprid has become one of most extensively used insecticides for both crop protection and animal health applications. As other classes insecticides, resistance to neonicotinoids is a significant threat been identified several pest species, including brown planthopper, Nilaparvata lugens , major rice many parts Asia. In this study, radioligand binding experiments have conducted whole-body membranes prepared from imidacloprid-susceptible imidacloprid-resistant strains N. . The results reveal much higher level [ 3 H]imidacloprid-specific susceptible strain than resistant (16.7 ± 1.0 0.34 0.21 fmol/mg protein, respectively). With aim understanding molecular basis resistance, five nAChR subunits (Nlα1–Nlα4 Nlβ1) cloned .A comparison subunit genes imidacloprid-sensitive populations single point mutation at conserved position (Y151S) two subunits, Nlα1 Nlα3. A strong correlation between frequency Y151S demonstrated by allele-specific PCR. By expression hybrid nAChRs containing α rat β2 evidence was obtained that demonstrates responsible substantial reduction specific H]imidacloprid binding. This study provides direct occurrence target-site neonicotinoid insecticide.