Essential role for Gα 13 in endothelial cells during embryonic development

• DOI: 10.1073/pnas.0503326102
• Publication Date: 2005-05-27T00:34:09Z
• Authors: Kathleen M. Ruppel; David Willison; Hiroshi Kataoka; Alice Wang; Yao-Wu Zheng; Ivo Cornelissen; Liya Yin; Shan Mei Xu; Shaun R. Coughlin
• Keywords: Knockout mouse; Gene knockout; Gene targeting

Toward identifying the roles of protease-activated receptor-1 (PAR1) and other G protein-coupled receptors important for vascular development, we investigated role Gα 13 in endothelial cells mouse embryo. LacZ inserted into α exon 1 was highly expressed at midgestation. Endothelial-specific knockout embryos died embryonic days 9.5–11.5 resembled PAR1 knockout. Restoration expression by use a Tie2 promoter-driven transgene rescued development endothelial-specific as well day 9.5 phenotype conventional knockouts; transgene-positive -/- developed several beyond their transgene-negative littermates then manifested previously uncharacterized that included intracranial bleeding exencephaly. Taken together, our results suggest critical during place candidate mediator signaling this process, reveal cell types mammalian