Production of infectious human papillomavirus independently of viral replication and epithelial cell differentiation

• DOI: 10.1073/pnas.0504020102
• Publication Date: 2005-06-16T00:25:46Z
• Authors: Dohun Pyeon; Paul F. Lambert; Paul Ahlquist
• Keywords: Bovine papillomavirus; Papillomaviridae

Papillomaviruses are small DNA viruses that associated with benign and malignant epithelial lesions, including >95% of cervical cancers ≈20% head neck cancers. Because papillomavirus replication virion production tied to cell differentiation, infectious has been limited cumbersome organotypic cultures mouse xenografts. Consequent difficulties in obtaining useful amounts wild-type or mutant human (HPV) virions have greatly studies on many aspects biology. To overcome these limitations, we developed a system encapsidate the full-length papillomaviral genome into virions, independently viral differentiation. This transient-transfection-based produces >1,000 times more virus per culture dish than much labor-intensive culture. Furthermore, show this method allows facile generation particles containing wild-type, mutant, chimeric genomes, overcoming barriers studying facets replication, host interactions, vaccine drug development, which by insufficient availability virions.